Topic:Cold and controlled nanoparticle beams for single particle diffractive imaging

Speaker：Amit K. Samanta

Report Time：8.25（Wednesday）17:00

Report Location：Tecent Meeting https://meeting.tencent.com/dm/i5A5nzpo8j2q  ID：187429140

Hosted by: Institute of Atomic and Molecular Physics

Introduction：

Amit  K. Samanta obtained his B.Sc. and M.Sc. degrees in Chemistry from the  University of Calcutta, India, and his Ph.D. from the Indian Association  for the Cultivation of Science (IACS), India. He worked as a  postdoctoral researcher at University of Southern California, USA,  during 2011--2016. From 2016, he is working as a research scientist at  Deutsches Elektronen-Synchrotron DESY, Germany. Currently, Amit is the  team leader for COMOTION project at CFEL, DESY.

Abstract：

Single-particle  diffractive imaging (SPI) is emerging as a new technique for 3D imaging  of aerosolized nanoparticles at x-ray free-electron lasers (XFELs).  How- ever, one of the primary bottlenecks in realizing SPI is the  efficient delivery of isolated, reproducible target particles into the  x-ray focus. Here, I present novel approaches for the production of cold  and high density beams of broad variety of biological nanoparticles,  ranging from single domain proteins including membrane proteins to  multi-subunit protein complexes and molecular machines, designed for use  in XFEL experiments. This will also enable us to gain a better  understanding of the ultrafast dynamics across extended biological  systems. Fast cooling from room temperature to 10 Kelvin in sub 10  microseconds will help freezing phase-space distribution, enhancing rare  structures, and even trapping reaction intermediates.